PBMC Isolation Services
Sampled offers validated, scalable PBMC isolation services designed to support clinical research, biomarker discovery, and immunological studies. Utilizing a combination of automated systems and manual expertise, each sample is processed with precision to ensure high viability, full traceability, and downstream compatibility with multiomic workflows.
What is PBMC Isolation?
Peripheral blood mononuclear cell (PBMC) isolation refers to the separation of lymphocytes and monocytes from whole blood, typically using density gradient centrifugation.
This technique yields a concentrated population of immune cells that are commonly used in immunology research, biomarker analysis, drug development, and clinical studies. Isolated PBMCs are essential for a range of downstream applications, including cell-based assays, flow cytometry, and multiomic analysis. However, it can represent a time-consuming and cost-inefficient process with a relatively low success rate. Outsourcing PBMC isolation to an experienced provider like Sampled can overcome these challenges. Our fully automated processes are overseen by our highly skilled team, who ensure that high-quality PBMCs are isolated from every sample we receive and that every step of the process is as precise, accurate, and reproducible as the last.
Why Choose Sampled for PBMC Isolation?
Outsourcing PBMC isolation to Sampled provides a number of operational and scientific benefits, particularly for institutions managing high sample volumes or complex workflows.
Time and Cost Efficiency
PBMC isolation requires significant time, specialized training, and investment in equipment. By outsourcing this process, research teams can reallocate internal resources to higher-value activities while avoiding the capital expense of maintaining in-house capabilities.
High Success Rate and Quality Assurance
PBMC isolation is a sensitive process that can result in sample loss or poor recovery if not performed under optimal conditions. We apply extensive technical expertise, optimized protocols, and purpose-built infrastructure to deliver consistently high yields and cell viability. All samples are assessed post-isolation for cell count and purity to ensure quality benchmarks are met.
Both Manual and Automated Processes
Sampled supports both automated and manual PBMC isolation processes, depending on study requirements and batch sizes. Automation enables efficient, high-throughput processing, while manual workflows provide flexibility for smaller or more complex projects. This hybrid approach ensures a high degree of accuracy, scalability, and reproducibility across a wide range of use cases.
PBMC Isolation Applications
PBMC isolation comes with a broad array of potential applications, which are advantageous across diverse research settings.
- Disease identification
- Diagnostics
- Drug discovery
- Pharmacogenomics
- Clinical trials
- Research studies
- Biomarker discovery
- Population health studies
- Disease modeling
- Development modeling
How Does PBMC Isolation Work?
Sample Receipt
Our logistics team can arrange blood shipments with several courier services. They will deal with any issues that arise and provide the required equipment. Alternatively, you can organize shipping if you prefer. When we receive your whole blood samples, they will be processed immediately, keeping cells at optimal conditions to ascertain that sample integrity is protected for cell viability and function. All blood samples are accessioned using barcoded labels for full traceability.
Plasma and PBMC Isolation
The next step is to isolate PBMCs from whole blood samples. Cell isolation is carried out by our team of experienced cell biologists using well-established density gradient centrifugation protocols, which separates plasma, PBMCs, and red blood cells from whole blood samples. We use top-of-the-range equipment such as the Hamilton easyBlood and well-established, automated protocols that ensure an exceptionally high success rate and the production of high-quality plasma and PBMCs for further assessment.
PBMC Preservation, Storage, or Shipment
Once we have successfully generated high-quality, viable PBMCs from a whole blood sample, we preserve them as per our cell banking service, allowing us to make PBMCs available to customers whenever they need them. PBMCs can also be processed further to generate high-quality DNA, RNA, or protein samples suitable for our multiomics services, or cells or samples can be sent back to clients who wish to perform in-house analysis.
What Are the Input Requirements for PBMC Isolation?
For our PBMC isolation service, we can work with any whole blood sample, which can be collected using kits provided by our custom clinical and research kitting team or using your own sample collection tools.
| Item | Type |
|---|---|
| Sample Types | Whole Blood |
| Storage/Transport Conditions | Ambient |
| Workflow Time | 48 hours |
Find out more about our PBMC isolation services
Looking to isolate PBMCs from whole blood samples but don’t have the time, resources, or technical expertise? Look no further! Get in touch today to find out more from one of our cellular services experts.
