What Is Sampled’s Genotyping Array Service?
Sampled’s genotyping array service is a cutting-edge solution tailored for scientists seeking to investigate genetic variation.
Our service leverages the power of Single Nucleotide Polymorphisms (SNPs), ubiquitous bi-allelic nucleotide variants found throughout the genome, occurring at an approximate frequency of one in 1,000 base pairs.
These SNPs, found in diverse genomic regions including coding, non-coding, and intronic regions, influence transcription factor binding, gene splicing, protein folding, and various other genetic elements. They play a crucial role in shaping genetic diversity and driving genome evolution, making them valuable markers for identifying genes linked to complex diseases. Genotyping allows scientists to explore genetic variation from single nucleotide variants to copy number variants and substantial structural changes within DNA.
Sampled’s genotyping array service employs pre-designed or custom array plates sourced from Thermo Fisher or Illumina platforms, bolstered by validated genomic content extracted from comprehensive genomic databases. By integrating complete reagent kits, innovative data analysis tools, and an automated workflow, our experienced team of scientists can provide you with a seamless, reliable, and cost-effective solution for all your genotyping requirements.
Why Use Sampled’s Genotyping Array Services?
Comprehensive, Reliable Genotyping
Genotyping arrays enable scientists to explore a broad range of genetic variants, such as single nucleotide variants, copy number variants, and structural changes in DNA. Our genotyping arrays provide the maximum genomic coverage of known and novel SNPs and indels (human only) and encompass coding and non-coding regions, thereby providing customers with a broad overview of large and small genetic variants within their sample of interest and a deeper understanding of disease etiology on a molecular level. Moreover, we utilize automated workflows to ensure our genotyping services are completed with a high degree of accuracy and reproducibility for reliable genotyping data that you can trust.
Our genotyping services can be tailored to suit your project-specific experimental or clinical requirements. We employ pre-designed and custom array plates from Thermo Fisher and Illumina platforms, providing flexibility to work for the specific needs of our clients. Moreover, we have the capabilities to work with a range of sample types and can work with externally produced DNA or extract it ourselves in-house. On top of that, we offer data analysis services to help you get the most out of your data once the genotyping is complete.
Cost- and Time-Effective
We understand that the capital costs of the required equipment, maintenance, and expertise for genotyping remain considerable. Outsourcing genotyping services to Sampled offers a cost-effective way to analyze all the variants of an organism’s genome without the high costs of equipment, maintenance, and bioinformatics support required, so you only pay for what you use. Moreover, our dedicated team and streamlined workflows enable us to offer rapid turnaround times, accelerating your research processes and driving discoveries.
Data Analysis Support
The vast range of capabilities across Sampled’s teams allows us to offer a fully end-to-end service. This means that our support doesn’t stop when the genotyping is complete: we can continue to work with you to support your data analysis needs, utilizing the expertise of our knowledgeable bioinformatics team.
Genotyping Array Applications
Genotyping provides comprehensive genome coverage and unparalleled alignment with established reference population databases. Genotyping allows scientists to explore genetic variants such as single nucleotide variants, copy number variants, and large structural changes in DNA. SNP analysis is significant for a broad range of potential applications including, but not limited to:
- Disease identification
- Drug discovery
- Research studies
- Biomarker discovery
- Population health studies
- Personalized medicine
How Does Sampled’s Genotyping Arrays Service Work?
Here at Sampled, we have the capability to process whole blood and saliva samples for genotyping arrays. Upon receiving samples, our in-house sample processing team processes these, extracting high-quality DNA from the samples and performing quality control checks on the extracted DNA. If we receive externally produced DNA, our team will perform the necessary quality control checks to ensure that only the highest quality DNA is used to generate high-quality data.
Once high-quality DNA has been extracted and quality checked, our scientists perform genotyping assays using Thermo Fisher or Illumina platforms. With the Thermo Fisher platform, the workflow is fully automated using the GeneTitan Multi-Channel instrument. With the Illumina platform, the workflow is partially automated and utilizes the iScan instrument. Both workflows employ automated liquid handling systems. The general steps to process the target DNA include amplification, fragmentation, precipitation, resuspension, hybridization, staining, and scanning.
The final step of any genomics-based workflow is data analysis. Following scanning, data files are generated for analysis. Our Bioinformatics Team will run quality checks on the raw genotyping data to ensure the workflow has been successfully completed producing high-quality data. If no further data analysis support is required, clients are provided with the raw data. Alternatively, our expert bioinformaticians can perform further data analysis using custom analysis pipelines to help you get the most out of your data.
What Are the Input Requirements for Genotyping Arrays?
|High-quality genomic DNA sourced from whole blood or saliva is required.
We can extract DNA from whole blood or saliva samples or work with externally produced DNA.
|Contact our team for specific details
|The assay requires samples to be normalized to a concentration of 20 -40 ng/µL for whole blood DNA and 40-75 ng/µL for saliva DNA in a volume of 30-50 µL.
All DNA should have an absorbance reading (260/280) of 1.7-2.0.
|1-2 weeks (dependent on the project size)
Find out more about our genotyping array services
Are you interested in learning more about the genotyping array services we offer here at Sampled? Do you have a project you’d like to discuss with one of our experts? Get in touch today to find out more and receive a bespoke quote for your project!