PacBio

Overview of PacBio Technology:

Sampled is proud to be a Certified Service Provider of PacBio HiFi long-read sequencing technology. The mission of PacBio is enabling the promise of genomics to better human health. Through this DNA/RNA Sequencing service Sampled is providing scientists with some of the world’s most advanced sequencing technology that enables insights to better understand biology and human health.¹

HiFi long-read sequencing is recognized as the most accurate and complete sequencing technology:

• PacBio HiFi offers 21× fewer errors vs alternative technology, >6× fewer errors vs Illumina in the Precision FDA benchmarking of total errors (SNV + indel + SV)
• Read lengths up to 25 kb allow you to span large structural variants and challenging repetitive regions, and to sequence full-length transcripts
• Epigenetics can be explored through direct detection of base modifications during sequencing

Which Long-Read Sequencing Methods are available?

Sampled can provide clients access to a range of Hi-Fi long-read sequencing methods to further their research and benefit from the PacBio sequencing technology, including:

Whole genome sequencing

Complete and accurate resolution through unbiased, single-molecule reads of up to 25 kb, a median read accuracy of >99.9% (Q30). No reads are under Q20.

RNA sequencing

Characterize the full diversity of the transcriptome including full-length isoforms.

AAV sequencing for Gene Therapy

High accuracy and complete visibility are critical to the success of novel vector discovery, vector design, and manufacturing quality control for gene therapy products.

Targeted sequencing

If you are using PCR, hybridization-capture, or amplification-free enrichment, HiFi sequencing allows you to discover and detect the full breadth of variants in targeted regions of the genome and transcriptome.

Complex Populations

Build pangenome references

Epigenetic

Use 5-base HiFi sequencing to study epigenetics of humans and other eukaryotes. Measure 5mC methylation at CpG sites directly from the sequencing instrument without the need for bisulfite treatment or other special library preparation methods.  Build a more comprehensive picture of how microbes use epigenetic modification for immune evasion by characterizing DNA modifications and methyltransferase recognition motifs for 4mC and 6mA.

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What Application Areas are Suitable?

This leading DNA sequencing technology is highly suited to a range of industries including:

Oncology Research

Accurate long reads allow you to uncover novel isoforms, fusions, and structural variants with unmatched accuracy.

Agriculture

HiFi technology gives plant and animal scientists access to actionable genomic insights that other technologies cannot provide.

Microbial Genomics

• Track the evolution of viral populations
• Resolve bacterial strains, serotypes, and plasmids
• Obtain improved gene discovery with reliably closed highly accurate bacterial genomes.
• Distinguish community composition at the strain level to correlate specific species to difference in metagenome function or systemic outcomes.
• Connect metagenome community functions by using closed and high-quality MAGs to align metagenome community functions to specific strains.

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Which PacBio Platform is Available?

Sampled is proud to be running both the PacBio Revio and the Sequel IIe which provide the following benefits:

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What does the PacBio platform look like in a Sampled lab workflow?

Working with Sampled to access PacBio technology means that researchers can benefit not just from the sequencing technology but the wider Sampled services which include the; Storage, Management, Analysis, Research and Transport of biosamples.

1. Samples collected as per Sampled management guidelines
2. Samples sent to Sampled for bioprocessing and library prep
3. Sample analysis using the PacBio Sequel IIe & SMRT Cell
4. Reporting sent to Client via informatics platform
5. Samples stored in Sampled biorepository for future access