Peripheral Blood Mononuclear Cell (PBMC) Isolation
Sampled’s PBMC isolation service means you will always have a reliable and validated stock of PBMCs. We offer both manual and automated protocols to improve scalability while reducing error for a high-throughput, time-efficient service you can trust.
What is PBMC Isolation?
This service allows researchers to prepare and isolate PBMCs from whole blood through density gradient centrifugation. This process involves the separation of PBMCs from other blood cells, such as red blood cells and granulocytes.
Necessary for a broad range of downstream applications such as biomarker discovery, drug discovery, oncology, and clinical trials, PBMC isolation is vital to many labs. However, it can represent a time-consuming and cost-inefficient process with a relatively low success rate. Outsourcing PBMC isolation to an experienced provider like Sampled can overcome these challenges. Our fully automated processes are overseen by our highly skilled team, who ensure that high-quality PBMCs are isolated from every sample we receive and that every step of the process is as precise, accurate, and reproducible as the last.
Why Use Sampled’s PBMC Isolation Services?
Outsourcing your PBMC isolation to Sampled comes with a whole host of advantages for our clients, including:
Time- and Cost-Efficiency
Outsourcing inherently time-consuming processes like PBMC isolation can greatly enhance researcher productivity. Moreover, the investment costs for the required specialized equipment can be high. By outsourcing PBMC isolation to Sampled, researchers will have more time and resources to focus on other aspects of their research.
High Success Rate
PBMC isolation is prone to failure and often results in wasted researcher time, laboratory resources, and precious samples. Moreover, achieving a fast turnaround time for high volumes of samples can be challenging, but at Sampled, we have the necessary expertise, resources, and facilities to accomplish this.
At Sampled, we have a vast amount of technical expertise, which means we have the capacity to process PBMCs with an extremely high success rate, so you can have peace of mind when sending us your precious samples. Following cell isolation, all PBMC samples will be assessed in terms of yield and purity to ensure the same high standards are always met.
Manual and Automated Processes
Our PBMC isolation services involve the integration of manual and automated processes, depending on the specific needs of the client and project. Our automated processes are great for high-scale projects, as they improve our throughput, meaning we can process more samples, more quickly. Moreover, integrating manual and automated workflows allows us to isolate PBMCs with a high degree of accuracy, precision, and reproducibility, producing high-quality cells you can trust.
PBMC Isolation Applications
PBMC isolation comes with a broad array of potential applications, which are advantageous across diverse research settings.
- Disease identification
- Diagnostics
- Drug discovery
- Pharmacogenomics
- Clinical trials
- Research studies
- Biomarker discovery
- Population health studies
- Disease modeling
- Development modeling
How Does PBMC Isolation Work?
Sample Receipt
Our logistics team can arrange blood shipments with several courier services. They will deal with any issues that arise and provide the required equipment. Alternatively, you can organize shipping if you prefer. When we receive your whole blood samples, they will be processed immediately, keeping cells at optimal conditions to ascertain that sample integrity is protected for cell viability and function. All blood samples are accessioned using barcoded labels for full traceability.
Plasma and PBMC Isolation
The next step is to isolate PBMCs from whole blood samples. Cell isolation is carried out by our team of experienced cell biologists using well-established density gradient centrifugation protocols, which separates plasma, PBMCs, and red blood cells from whole blood samples. We use top-of-the-range equipment such as the Hamilton easyBlood and well-established, automated protocols that ensure an exceptionally high success rate and the production of high-quality plasma and PBMCs for further assessment.
PBMC Preservation, Storage, or Shipment
Once we have successfully generated high-quality, viable PBMCs from a whole blood sample, we preserve them as per our cell banking service, allowing us to make PBMCs available to customers whenever they need them. PBMCs can also be processed further to generate high-quality DNA, RNA, or protein samples suitable for our multiomics services, or cells or samples can be sent back to clients who wish to perform in-house analysis.
What Are the Input Requirements for PBMC Isolation?
For our PBMC isolation service, we can work with any whole blood sample, which can be collected using kits provided by our custom clinical and research kitting team or using your own sample collection tools.
| Item | Type |
|---|---|
| Sample Types | Whole Blood |
| Storage/Transport Conditions | Ambient |
| Workflow Time | 48 hours |
Find out more about our PBMC isolation services
Looking to isolate PBMCs from whole blood samples but don’t have the time, resources, or technical expertise? Look no further! Get in touch today to find out more from one of our cellular services experts.
