What is Sampled’s Transcriptome Profiling Array Service?
Sampled’s transcriptome profiling array service offers a comprehensive solution for scientists seeking to understand gene expression patterns in specific target populations.
This service focuses on the analysis of mRNA transcripts associated with your areas of interest, whether they pertain to diseases, inheritance, or cell growth, among other possibilities.
Our process begins by reverse transcribing mRNA, converting it into complementary DNA (cDNA). This cDNA is then hybridized with custom-designed array plates featuring probes or nucleic acids of known sequences. These arrays are tailored to your research project’s unique requirements, ensuring precise data collection.
By employing our transcriptome profiling array service, you gain valuable insights into the correlation between the genes within your sample and those associated with the population you are studying. This approach provides a deeper understanding of gene expression levels, facilitating meaningful scientific discoveries.
Why Use Sampled’s Transcriptome Profiling Array Services?
Comprehensive, Accurate Transcriptome Profiling
Transcriptome profiling arrays enable scientists to explore gene expression through mRNA transcripts associated with target populations of interest. Our kits, instrumentation, and software consistently deliver data of high quality and accuracy. Moreover, we utilize automated workflows to ensure our transcriptome profiling services are completed with a high degree of accuracy and reproducibility for reliable transcriptome profiling data that you can trust.
Our transcriptome profiling services can be tailored to suit your project-specific research or clinical requirements. Individual cartridge arrays are available for small projects. In addition, microarray plates are available in 96- and 384- formats offering high-throughput options, making this assay versatile and useful among assays in its class. This flexibility contrasts with other methods that impose fixed costs for a set number of arrays, whether they are needed or not. Moreover, we can work with a range of sample types and can work with externally produced RNA or extract it in-house. On top of that, we offer custom data analysis services to help you get the most out of your data once the transcriptome profiling is complete.
Cost- and Time-Effective
We understand that the capital costs of the required equipment, maintenance, and expertise for genotyping remain considerable. Outsourcing transcriptome profiling services to Sampled offers a cost-effective way to analyze the expression levels of genes in any sample without the high costs of equipment, maintenance, and bioinformatics support, so you only pay for what you use. Moreover, our dedicated team and streamlined workflows enable us to offer rapid turnaround times, accelerating your research processes and driving discoveries.
Data Analysis Support
The vast range of capabilities across Sampled’s teams allows us to offer a full end-to-end service. This means that our support doesn’t stop when the transcriptome profiling arrays are complete: we can continue to work with you to support your data analysis needs, utilizing the expertise of our knowledgeable bioinformatics team.
Transcriptome Profiling Array Applications
Transcriptome profiling arrays can provide you with a wealth of information regarding gene expression. Gene expression analyses are at the center of biological research and clinical decision-making, meaning the range of applications for our transcriptome profiling array service is vast.
- Disease identification
- Drug discovery
- Research studies
- Biomarker discovery
- Population health studies
- Personalized medicine
How Does Sampled’s Transcriptome Profiling Array Service Work?
Here at Sampled, we have the capability to process whole blood and cell line samples for transcriptome profiling arrays. Upon receiving samples, our in-house sample processing team processes these, extracting high-quality RNA from the samples and performing quality control checks on the extracted RNA. If we receive externally produced RNA samples, our team will perform the necessary quality control checks to ensure that only the highest quality RNA is analyzed with our transcriptome profiling pipelines.
Transcriptome Profiling Arrays
Once high-quality RNA has been extracted and quality checked, our scientists perform transcriptome profiling assays using Thermo Fisher platforms. Staining and scanning are carried out using either the GeneChip® Scanner 3000 or the Gene Titan Multi-Channel instruments with automated liquid handling systems The general steps for the workflow include cDNA synthesis, amplification, fragmentation and labeling, hybridization, and scanning.
The final step of any genomics-based workflow is data analysis. Following scanning, data files are generated for analysis. Our Bioinformatics Team will run quality checks on the raw transcriptome profiling data to ensure the workflow has been successfully completed producing high-quality data. If no further data analysis support is required, clients are provided with the raw data. Alternatively, our expert bioinformaticians can perform automated data analysis using custom analysis pipelines to help you get the most out of your data.
What Are the Input Requirements for Transcriptome Profiling Arrays?
|High-quality RNA sourced from whole blood or cell lines is required.
We can extract RNA from whole blood or cell line samples or work with pre-extracted RNA.
|Contact our team for specific details
|The assay requires samples to be normalized to a concentration of 50 ng/µL in a volume of 30 µL.
|1-2 weeks (dependent on the array and number of samples)
Find out more about our transcriptome profiling array services
Are you interested in learning more about the transcriptome profiling array services we offer here at Sampled? Get in touch today to speak to an expert and receive a bespoke quote for your project!