Methylation Profiling
Investigate the methylation status of your samples’ DNA and obtain valuable insights into gene regulation and disease pathways by outsourcing your samples for methylation profiling to Sampled.
What Is Sampled’s Methylation Profiling Service?
Sampled’s methylation profiling service is designed to provide scientists with a powerful tool for studying DNA methylation and its implications across various research areas.
Aberrant DNA methylation has been linked to a wide range of disease processes, including cancer, obesity, and neurodegeneration. As a result, DNA methylation analysis offers valuable insights into gene regulation and facilitates the identification of potential biomarkers. Additionally, DNA methylation status can help researchers better understand how environmental factors such as drought and temperature extremes impact agrigenomics.
Our approach involves the use of microarrays from the Illumina platform, along with reagent kits, data analysis tools, and an automated workflow that incorporates iScan instruments. This comprehensive process enables high-throughput genome-wide methylation profiling studies, allowing scientists to investigate the significance of DNA methylation and gain novel insights into the functional consequences of epigenetic variations. Sampled’s methylation profiling service is here to support your research efforts and drive discoveries.
Why Use Sampled’s Methylation Profiling Service?
Comprehensive Quantitative Methylation Analysis
Methylation microarray technology is a proven approach to analyze DNA methylation. The arrays enable quantitative interrogation of selected methylation sites across the genome, offering high-throughput capabilities that minimize the cost per sample to study methylation as a biomarker for many diseases like cancer, Alzheimer’s, and epigenetic therapeutics.
Moreover, we utilize automated workflows to ensure our methylation profiling services have a high degree of accuracy and reproducibility for reliable data that you can trust.
Flexible
Our methylation profiling services can be customized to your project-specific research l or clinical requirements. We have the capabilities to work with a range of sample types and can work with externally produced DNA or extract it ourselves in-house from whole blood or saliva samples. On top of that, we offer data-analysis services to help you get the most out of your data once the methylation profiling is complete.
Cost- and Time-Effective
We understand that the capital costs of the required equipment, maintenance, and expertise for methylation profiling remain considerable. Outsourcing to Sampled offers a cost-effective way to analyze the methylation status of DNA in any sample without the high costs of equipment, maintenance, and bioinformatics support, so you only pay for what you use. Moreover, our dedicated team and streamlined workflows enable us to offer rapid turnaround times, accelerating your research processes and driving discoveries.
Data Analysis Support
The vast range of capabilities across Sampled’s teams allows us to offer a full end-to-end service. This means that our support doesn’t stop when the methylation profiling arrays are complete: we can continue to work with you to support your data analysis needs, utilizing the expertise of our knowledgeable bioinformatics team.
Methylation Profiling Applications
DNA methylation is essential for normal development, and it plays a crucial role in several processes that, when dysregulated, can lead to disease. As a result, examining the methylation status of DNA samples can be pivotal in a broad range of research and clinical studies.
- Disease identification
- Diagnostics
- Drug discovery
- Pharmacogenomics
- Clinical trials
- Research studies
- Biomarker discovery
- Population health studies
- Novel genome assembly
- Agrigenomics
- Disease modeling
- Development modeling
How Does Sampled’s Methylation Profiling Service Work?
Sample Preparation
Here at Sampled, we can process whole blood and saliva samples for methylation processing. Upon receiving samples, our in-house analytical sample processing team processes these, extracting high-quality DNA from the samples and performing quality control checks on the extracted DNA. If we receive externally produced DNA samples, our team will perform the necessary quality control checks to ascertain that only the highest quality DNA enters methylation profiling pipelines.
Genotyping Arrays
Once high-quality DNA has been extracted and quality checked, our scientists perform methylation profiling assays using Illumina platforms in combination with the iScan instruments and automated liquid handling systems. The general steps to process the target DNA include amplification, fragmentation, hybridization, staining, and scanning.
Data Analysis
From the scanning of the arrays, data files are generated for analysis. First, raw methylation profiling data will be quality-controlled to ensure the workflow has successfully produced high-quality data. If no data analysis support is required, clients receive the raw data for in-house analysis. Alternatively, our expert bioinformaticians can perform automated data analysis using custom analysis pipelines to help you get the most out of your data.
What Are the Input Requirements for Sampled’s Methylation Profiling Service?
| Item | Type |
|---|---|
| Sample Types | High-quality genomic DNA sourced from whole blood or saliva is required. We can extract DNA from whole blood or saliva samples or work with externally produced DNA. |
| Storage/Transport Conditions | Contact our team for specific details |
| Input Requirements | The assay requires samples to be normalized to a concentration of 20-40 ng/µL for whole blood DNA and 40-75 ng/µL for saliva DNA; in a volume of 30-50 µL. All DNA should have an absorbance reading (1260/280) of 1.7-2.0. |
| Workflow Time | 1-2 weeks |
Find out more about our methylation profiling services
Are you interested in learning more about the methylation profiling services we offer here at Sampled? Get in touch today to speak to an expert and receive a bespoke quote for your project!
