iPSC Quality Control
Here at Sampled, we offer robust quality control checks on the stem cells we produce as part of our iPSC reprogramming and gene editing services. We guarantee that our clients will receive the precise clones they need thanks to our iPSC quality control service.
What Is iPSC Quality Control?
Quality control (QC) is an essential component of our induced pluripotent stem cell (iPSC) services pipeline here at Sampled.
iPSC QC involves examining characteristics that represent key stem cell qualities, such as alkaline phosphatase staining and embryoid body formation. iPSC QC also includes more generic cell line quality control tests like mycoplasma testing and cell viability. Here at Sampled, our robust iPSC QC service means that our clients receive the best possible stem cell products for their research.
Why Use Sampled’s iPSC Quality Control Service?
Expertise
At Sampled, we have vast experience generating and testing stem cells. Our platform is set up to process a wide variety of parameters to ensure you are getting a product you can trust. Our seasoned team of experts is ready to tackle any quality control requirements you may have.
Time- and Cost-efficient
iPSC QC represents a set of methods that can be costly to develop in-house in terms of the money and time required to train technicians and troubleshoot issues. By outsourcing your iPSC QC needs to Sampled, you can use your time and resources to focus on other things while ensuring your sample is in the hands of global experts using state-of-the-art instruments.
Guaranteed
At Sampled, we guarantee that our iPSC QC will provide clients with their desired clones. Our team of experts will continue to screen clones until we have the right ones for our clients.
Applications of iPSC Quality Control
The applications for our iPSC QC service are research-only, and we work with both non-profit and for-profit institutes. Applications for this service include:
- Research studies
- Biomarker discovery
- Population health studies
- Agrigenomics
- Disease modeling
- Development modeling
How Does Stem Cell Quality Control Work?
Client Parameters
The first step in iPSC QC is to determine what level of QC our client wants and that best suits their needs. We offer standard QC tests and further optional QC tests.
Gene Editing QC
After our editing process is complete, we perform single-cell screening using Sanger sequencing to determine that the desired edit has been achieved and is in the correct genomic location. After cell proliferation, we perform next-generation sequencing to ensure that the population is homogeneous. Some areas of the genome are more prone to aberrant editing than others, so we screen these regions to ensure they have not been edited.
iPSC QC
After editing QC, we perform iPSC QC to ensure that our expanded cell line maintains iPSC characteristics. Our standard QC includes:
- Sterility testing
- Mycoplasma testing
- Viability
- Stem cell marker FACS
- Alkaline phosphatase staining
Further optional QC steps we offer include:
- Karyotyping
- Embryoid body formation (scorecard for germ layer differentiation)
- Pluritest array
- Sendai persistence (for blood-derived iPSCs)
What Are the Input Requirements for iPSC Quality Control?
| Item | Type |
|---|---|
| Sample Types | iPSCs generated in-house or sent by clients |
| Storage/Transport Conditions | Frozen |
| Input Requirements | 1 million cells/vial |
Find out more about our iPSC quality control service
Looking to outsource your iPSC reprogramming and gene editing but have concerns about QC? Get in touch with one of Sampled’s experts today to discover how we can meet your iPSC QC needs.
