iPSC Differentiation into Neuronal Cells
iPSCs can be used as powerful tool in regenerative medicine, drug discovery, therapeutic disease modelling, and to study development.
Speak to an expert
How does the service work?
We are happy to announce that at Sampled, in addition to iPSC reprogramming and gene editing services, now offer iPSC-derived neuronal progenitor cell (NPC) differentiation services. NPCs are a progenitor population of cells that can differentiate into neuronal populations and can be used for studying physiology, disease phenotypes and drug testing.
Using a robust and reproducible method developed by Dr. Sally Temple at Neuracell, iPSC-derived NPCs are generated in culture using guidance factors such as dual SMAD inhibition, to recapitulate the development of the forebrain and create NPCs that can be differentiated into forebrain neurons.
NPCs can be requested 3 ways
1
NPCS from a limited number of disease phenotypes are available off the shelf (Table 1 – cell lines subject to change).
- Researchers will receive 2 x 106 cells per vial
2
iPSC available from the NINDS Cell and Human Data Repository (NHCDR; found at stemcells.nindsgenetics.org) and the NeuraCell Core Facility (neuracell.org/cell-supply/stem-cells) can be used to generate NPCs.
- Researchers will receive 16-20 vials containing 2 x 106 cells per vial
- Turn around time of 3-4 months for up to 10 individual cell lines
3
Arrangements can be made for clients to provide iPSC of interest.
- Researchers will receive 16-20 vials containing 2 x 106 cells per vial
- Turn around time of 3-4 months for up to 10 individual cell lines
All our cells undergo rigorous quality control tests that include:
- Proliferation assays to demonstrate proliferation of NPCs
- qPCR to confirm expression of GAPDH, FOXG1, PAX6 and lack of expression of DLX2 and NKX2.1
- IHC to demonstrate expression of PAX6, Nestin and SOX2 and lack of expression of SOX10
Tau Consortium iPSC lines
Table 1: Select Tau Consortium iPSC lines already validated for NPC production (see Karch et al, 2019)
| Cell Line | Mutation | Genotype |
|---|---|---|
| F11362.1 | R406W | WT/R406W |
| F11362.1d1C11 | R406W | WT/WT |
| F11362.1d1F10 | R406W | WT/R406W |
| F12436.3 | P301L/S | WT/WT (P301L family) |
| F12442.5 | PSEN1 | WT/WT |
| FTD-38-C1 | A152T | WT/A152T |
| FTD-T-C2 | A152T | WT/A152T |
| GIH-131-C1 | PSP | PSP |
| GIH-131-C2 | PSP | PSP |
| GIH-143-C3 | R406W | R406W homozygote |
| GIH-161-C1 | WT | WT/WT |
| GIH-161-C3 | WT | WT/WT |
| GIH-161-C4 | WT | WT/WT |
| GIH-6-C1d1A02 | V337M | WT/V337M |
| GIH-6-C1d1E11 | V337M | WT/WT |
| GIH-7-C2d2A01 | V337M | WT/V337M |
| GIH-7-C2d2B12 | V337M | WT/WT |
| GIH-7-C3 | V337M | WT/V337M |
| GIH-92-C1 | PSP | PSP |
| GIH-92-C2 | PSP | PSP |
| GIH-92-C3 | PSP | PSP |
| TAU-1225-C3 | A152T | WT/A152T |
Enquire
All NPCs can be shipped globally. If you would like to get in touch with us, please use the form below:
