Targeted Sequencing
Targeted sequencing at Sampled allows you to hone in on your specific genomic region of interest and gain detailed and accurate insights. Sampled’s team of experts is ready to help you design, perform, and analyze your targeted sequencing experiment.
What Is Targeted Sequencing?
Targeted sequencing is genome sequencing but with a focus on a specific region or regions of interest.
This is different from whole-genome approaches, providing a sequencing strategy tailor-made to your specific needs. Targeted sequencing provides the client with maximum specificity while minimizing costs associated with larger sequencing projects.
Why Use Sampled’s Targeted Sequencing Services?
Cost-Efficient
Whole-genome sequencing is an incredibly powerful technology, but it comes with a relatively high cost and takes more time to complete. By choosing Sampled’s targeted sequencing services, you will pay only for what you need and have a team of experts helping you every step of the way.
Fully Customized
Targeted sequencing is fully customized to your needs. Whether you want to focus on the promoter region of a single gene or map all the components of a broad signaling pathway, Sampled is happy to help with whatever you need.
Expertise
By trusting your targeted sequencing project to Sampled, you will be collaborating with a team of global experts with a vast array of experience. Sequencing studies can be difficult to design, especially if it’s your first time. The Sampled team is ready to help you take your research to the next level.
Data Analysis
Sampled provides an expert data analysis service after the sequencing is complete. This is a great option to save money and time, especially if you don’t have the relevant in-house expertise.
Targeted Sequencing Applications
Targeted sequencing provides a detailed analysis of your specific genomic region of interest, and the list of potential applications is vast. Get in touch with the Sampled team to find out if targeted sequencing is the right approach for your project!
- Clinical applications
- Disease identification
- Diagnostics
- Pharmacogenomics
- Research studies
- Population health studies
- Cancer research
- Infectious disease research
- Novel genome assembly
- Agrigenomics
- Trait selection
How Does Targeted Sequencing Work?
Sample Preparation
The Sampled team is prepared to process a wide variety of sample types from clinical and research settings. Our sample processing team takes great care with your samples, ensuring that high-quality DNA is extracted. Stringent quality assurance is performed to confirm DNA quality before it is carried forward for sequencing pipelines.
Library Preparation
Library preparation is the process of preparing DNA samples for sequencing workflows. It involves fragmentation, end-repair, and covalent linking of genomic DNA (gDNA) to adapters (small, artificial DNA sequences) to produce a library of appropriately sized fragments, allowing for downstream processing and sample identification.
Sequencing
For targeted sequencing, the Sampled team offers both Illumina’s advanced NovaSeq 6000 and NovaSeq X-Plus sequencing systems, as well as PacBio HiFi sequencing using the Revio and Sequel IIe systems, depending on your needs. Sampled’s team of experts will help you choose the best platform that suits your project’s needs.
Data Analysis
The final step of the targeted sequencing workflow is data analysis. Quality control checks are performed to ensure the data is of high quality. Then data is sent to the client or analyzed by Sampled’s in-house expert bioinformaticians. Outsourcing your data analysis to Sampled ensures you are getting high-quality, unbiased analysis and getting the most out of your data.
What Are the Input Requirements for Targeted Sequencing?
The input for targeted sequencing is DNA which can be extracted from a diverse range of sample types. Sampled’s team of experts handles sample processing and DNA extraction before library preparation and sequencing. We work with samples derived from several organisms, including humans, mice, and rats.
| Item | Type |
|---|---|
| Sample Types | High-quality DNA sourced from blood, serum, saliva, tissue biopsies, hair, nails, stool, urine, semen, and cell line samples/pellets. We also work with pre-extracted DNA. |
| Storage/Transport Conditions | Pre-extracted DNA should be transported at 4°C (or as close to the previous storage conditions as possible). For information on other tissue types, please contact a member of our team. |
After extraction, your sample will be subjected to quality controls, ensuring that only high-quality DNA is sent forward for analysis.
Find out more about our targeted sequencing services
Looking to get sequencing data but only for a small subset of genes? Sampled’s targeted sequencing service could be exactly what you are looking for. Get in touch with one of our experts today and receive a customized quote for your project!
