Multiomics
Sampled’s comprehensive multiomics services encompass sample processing and extraction, sequencing (DNA, RNA, proteomics), PCR, microarrays for in-depth variation analysis, and tailored bioinformatics, promoting seamless research integration.
What Are Sampled’s Multiomics Services?
Robust multiomics capabilities are at the heart of Sampled’s offerings as the world’s only global integrated analytical biorepository (GIAB).
Multiomics is the focal point where our robust logistics, kitting, sample storage, and management systems deliver our client’s samples to our team of analytical experts. Full integration of the analytical workflow within Sampled’s GIAB capacities ensures a streamlined research process from sample receipt and storage to analysis and custom data reporting.
Analytical Sample Processing
Sampled offers comprehensive sample processing services, including extraction of high-quality RNA and DNA, fractionation, normalization, stringent quality control (QC) checks, and efficient aliquoting, ensuring precise and efficient handling of diverse sample types for various downstream applications.
Sampled’s analytical sample processing workflow covers extraction, fractionation, normalization, sample processing QC, and aliquoting. Samples are accepted at room temperature or frozen, depending on the sample type. Rigorous QC ensures sample suitability for downstream analysis. Fractionation is conducted using an automated process, which also enables precise aliquoting of samples. Normalization parameters are tailored to sample characteristics and workflow demands. QC checks are performed for DNA, RNA, plasma, and serum. After these steps, samples can be directed for analysis, storage, or shipping, with logistics managed by Sampled’s expert team.
Sequencing
Sampled’s sequencing services are vital for academic, industrial, and clinical research. We offer whole genome sequencing (WGS), whole exome sequencing (WES), targeted sequencing, Sanger sequencing, RNA sequencing, methylation sequencing, and protein biomarker discovery. Our customizable services provide detailed insights and expert support for various research and diagnostic needs.
Sampled offers a comprehensive range of sequencing services, including WGS, WES, targeted sequencing, Sanger sequencing, RNA sequencing, methylation sequencing, and protein biomarker discovery. Our experienced team ensures high-quality DNA extraction and library preparation for WGS and WES, using advanced platforms like Illumina‘s NovaSeq and PacBio‘s Revio. Data analysis, with QC, can be performed in-house or shared with clients. Targeted sequencing is available with flexible platform options. Sanger sequencing is performed and analyzed by in-house experts. RNA sequencing includes robust QC and platform flexibility. Methylation sequencing uses Illumina’s NovaSeq and PacBio’s HiFi platform. Protein biomarker discovery leverages the latest technology from Olink, with data analysis performed by Sampled experts.
Microarrays
Sampled offers genotyping, transcriptome profiling, and methylation profiling arrays. Genotyping arrays assess genetic diversity and disease markers via SNPs, transcriptome arrays study gene expression, and methylation arrays explore DNA methylation’s role in gene regulation. Customized array plates enable diverse research for health and disease discoveries.
Sampled offers comprehensive sample preparation and microarray services. Microarrays are performed using advanced platforms combined with automated instruments. After array scanning, data files are generated, quality-controlled, and can be sent to our clients. Alternatively, our expert bioinformaticians offer automated data analysis to maximize data insights, providing clients with valuable results and support throughout the process.
PCR
Sampled offers comprehensive quantitative PCR (qPCR) and droplet digital (ddPCR) services, providing critical insights into gene expression and detecting structural variants. qPCR is highly sensitive and enables real-time analysis of specific DNA sequences and absolute target DNA quantification. ddPCR, with unparalleled sensitivity, detects low-abundance targets, ensuring precision in genetic research, clinical diagnosis, and environmental science.
At Sampled, our PCR services encompass both qPCR and ddPCR. For qPCR, we uphold stringent DNA and RNA handling standards, ensuring high-quality input through rigorous QC. Whether processing RNA or raw samples, we perform reverse transcription, carry out quality control, and normalize DNA concentration for optimal amplification detection. The qPCR platform is flexible, accommodating different chemistries, and supports 96- and 384-well formats for high throughput. Similarly, in ddPCR, Sampled excels in diverse sample handling with meticulous droplet generation and analysis by expert bioinformaticians, providing comprehensive insights with statistical reliability.
Bioinformatics
Sampled’s team of bioinformaticians brings extensive expertise in data analysis and data formatting. Sampled’s bioinformatics platform integrates seamlessly with our other workflows and leverages the latest technology through Sampled’s partnership with leading sequencing technology providers like Illumina NovaSeq, PacBio Revio, and 10X Genomics. We offer tailored analysis and customized output, all while prioritizing data security, ensuring encryption, and compliance with regulatory standards such as Title 21 CFR part 11 and HIPAA. Sampled emphasizes client data ownership and confidentiality throughout our processes.
Sampled’s bioinformatics analysis includes secure data storage via SampledSphere, which offers extensive formatting flexibility. Data analysis is tailored to meet sequencing or array requirements, using cloud services like Illumina BaseSpace and DNAnexus, alongside an in-house pipeline. Our bioinformatics custom reporting service allows clients to specify data formats that best suit their requirements. If clients need guidance, our experts are ready to assist with any problems you may have.
Why Use Sampled’s Multiomics Services?
Expertise
Sampled’s dedicated team possesses diverse expertise in microarray analysis, sample processing, PCR, and sequencing services. Our professionals bring extensive experience to the table, enabling adept handling of a broad spectrum of sample types and research requirements. Whether your project delves into genomics, epigenomics, transcriptomics, or proteomics, our experts are well-prepared to provide comprehensive support. Our experience ensures the delivery of high-quality results, upholding global standards of precision and accuracy.
Quality Control
QC is fundamental to Sampled’s services. We prioritize rigorous QC procedures to guarantee the reliability and accuracy of data, whether from microarray or sequencing projects. Regardless of whether samples are processed by our team or yours, only the highest-quality samples advance for analysis.
Security
Data security is a top priority at Sampled. We employ encryption for data transfer and secure access through SampledSphere, ensuring strict compliance with Title 21 CFR part 11 and HIPAA regulations. Your data is only accessible to authorized individuals. We safeguard your data ownership, prohibiting third-party distribution or sale.
Flexibility
Sampled provides remarkable flexibility across all services, customizing analytical sample processing to meet specific project requirements. Whether you need pre-designed or customized array plates or a combination of manual and automated workflows, we adapt to your project’s complexity, timeline, and scale. Our flexibility extends to data analysis support, where we collaborate closely to align data analysis with research objectives, even analyzing data generated outside of Sampled. Our flexible solutions simplify your research processes.
Streamlined
Efficiency is a central tenet at Sampled. By entrusting your sample processing and analysis to us, you streamline the research process, eliminating many error-prone steps. Our in-house system ensures a streamlined chain of custody which promotes sample integrity, enhances security, and translates to significant time and cost savings, allowing you to focus resources on research questions with confidence.
Data Analysis Support
Sampled offers comprehensive support from initial sequencing to final data analysis. Our team of experienced bioinformaticians provides expert data analysis support, ensuring robust, reliable results meeting the highest scientific standards. Our commitment extends beyond raw data, as we aim to assist in deriving valuable insights from your research, whether in genomics, epigenomics, transcriptomics, or proteomics.
Multiomics Applications
Our multiomics platform consists of a wide range of techniques each with a wide range of potential applications.
These include:
- Disease identification
- Diagnostics
- Drug discovery
- Pharmacogenomics
- Clinical trials
- Research studies
- Biomarker discovery
- Population health studies
- Novel genome assembly
- Agrigenomics
- Disease modeling
- Development modeling
- Drug testing
- Mutation detection
- Copy number determination
- Genome edit detection
- Expert design assays
- Residual DNA quantification
- Library quantification
- Pathogen detection
- Gene expression analysis
- Absolute DNA quantification
- Fusion gene detection
Multiomics Input Requirements
At Sampled, we can extract DNA, RNA, and protein from a wide variety of tissue types from species including, but not limited to, human, mouse, and rat.
Tissues include:
- Blood
- Plasma
- Serum
- Saliva
- Tissue biopsies
- Hair
- Nail
- Stool
- Urine
- Semen
- Cell line samples/pellets
- Protein samples
- Isolated nucleic acids (DNA/RNA)
- Buccal swabs
- Environmental samples
Our expert bioinformatics team also processes raw sequencing data sent to us by our clients.
Find out more about Sampled’s multiomics services
Interesting in taking your research project to the next level with a multiomics project? Get in touch with one of Sampled’s experts today to discover how we can help leverage the power of multiomics to get the most out of your samples.
