Microarrays
Sampled offers genotyping, transcriptome profiling, and methylation profiling arrays for in-depth exploration of genetic, transcriptomic, and methylation variations in samples, fostering discoveries in health and disease research.
What Are Sampled’s Microarray Services?
Sampled’s microarray services encompass genotyping arrays, transcriptome profiling arrays, and methylation profiling arrays to enable scientists to explore variation in the genomes, transcriptomes, and methylation status of their samples of interest, driving insights and discoveries across health and disease scenarios.
Genotyping Arrays
Sampled offers a cutting-edge genotyping array service for scientists investigating genetic variation. Leveraging Single Nucleotide Polymorphisms (SNPs), common nucleotide variants found throughout the genome, this service helps researchers explore genetic diversity, including complex diseases’ genetic markers. Sampled uses pre-designed or customized array plates from trusted platforms like Thermo Fisher or Illumina, integrated with validated genomic content from comprehensive databases. With complete reagent kits, innovative data analysis tools, and an automated workflow, Sampled provides a seamless, dependable, and cost-effective genotyping solution.
Transcriptome Profiling Arrays
Sampled’s transcriptome profiling array service is a comprehensive solution for scientists studying gene expression patterns in specific populations. It involves analyzing mRNA transcripts related to various research areas, including diseases, inheritance, and cell growth. The process entails reverse transcribing mRNA into cDNA, which is then analyzed within custom-designed array plates. This tailored approach allows for precise data collection, providing valuable insights into gene correlations and expression levels and facilitating meaningful scientific discoveries in your research.
Methylation Profiling Arrays
Sampled’s methylation profiling service is a powerful tool for scientists studying DNA methylation and its impact across various research areas. It provides insights into gene regulation and biomarker identification, with links to diseases like cancer, obesity, and neurodegeneration. Additionally, it can help scientists understand how environmental factors affect agrigenomics. Using Illumina microarrays, reagent kits, and automated instruments, this service allows high-throughput genome-wide methylation profiling, facilitating research on epigenetic variations and functional consequences. Sampled’s service is dedicated to supporting research efforts and driving discoveries in this field.
Why Use Sampled’s Microarray Services?
Comprehensive, Reliable Profiling
All our microarray services offer comprehensive coverage and accurate data, thereby providing customers with a broad overview of large and small changes to SNPs, DNA, and RNA within their sample of interest and a deeper understanding of disease etiology on a molecular level. Moreover, we utilize automated workflows to ensure our microarray services are completed with a high degree of accuracy and reproducibility for reliable data that you can trust.
Flexible
All our microarray services can be tailored to suit your project-specific research or clinical requirements. We employ pre-designed and customized array plates from various platforms, so we have the flexibility to work to the specific needs of our clients. Moreover, we can work with a range of sample types and can work with externally produced DNA or RNA or extract them ourselves in-house. On top of that, we offer data analysis services to help you get the most out of your data once the microarrays are complete.
Cost- and Time-Effective
We understand that the capital costs of the required equipment, maintenance, and expertise for microarray profiling remain considerable. Outsourcing microarray services to Sampled offers a cost-effective way to analyze samples without the excessive costs of equipment, repairs, and bioinformatics support required, so you only pay for what you use. Moreover, our dedicated team and streamlined workflows enable us to offer rapid turnaround times, accelerating your research processes and driving discoveries.
Data Analysis Support
The vast range of capabilities across Sampled’s teams allows us to offer a fully end-to-end service. This means that our support doesn’t stop when the microarray data is complete: we can continue to work with you to support your data analysis needs, utilizing the expertise of our knowledgeable bioinformatics team.
Microarrays Applications
Sampled’s microarray services can facilitate a range of applications including, but not limited to:
- Disease identification
- Diagnostics
- Drug discovery
- Pharmacogenomics
- Clinical trials
- Research studies
- Biomarker discovery
- Population health studies
- Novel genome assembly
- Agrigenomics
- Disease modeling
- Development modeling
How Do Sampled’s Microarray Services Work?
Sample Preparation
Here at Sampled, we can process various samples for microarrays. For genotyping and methylation processing, we can process whole blood and saliva samples. For transcriptome profiling, we can process whole blood or cell line samples. Upon receiving samples, our in-house analytical sample processing team processes these, extracting high-quality DNA or RNA and performing quality control checks on the extracted samples. If we receive externally produced DNA/RNA samples, our team will perform the necessary quality control checks to ensure that only the highest quality nucleotides a microarray pipelines.
Microarrays
Once the high-quality DNA/RNA samples have been extracted and quality checked, our scientists perform microarray analysis using Thermo Fisher and Illumina platforms in combination with the GeneTitan, iScan, or GeneChip instruments and automated liquid handling systems. For all microarray workflows, the general steps to process the target DNA include amplification, fragmentation, hybridization, staining, and scanning.
Data Analysis
From the scanning of arrays, data files are generated for analysis. First, raw microarray data will be quality-controlled to ensure the workflow has successfully produced high-quality data. If no data analysis support is required, clients are provided the raw data for in-house analysis. Alternatively, our expert bioinformaticians can perform automated data analysis using custom analysis pipelines to help you get the most out of your data.
What Are the Input Requirements for Microarrays?
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| Item | Type |
|---|---|
| Sample Types | Genotyping and Methylation Profiling: High-quality genomic DNA sourced from whole blood or saliva is required. We can extract DNA from whole blood or saliva samples or work with externally produced DNA. Transcriptome Profiling: High-quality RNA sourced from whole blood or cell lines is required. We can extract RNA from whole blood or cell line samples or work with externally produced RNA. |
| Storage/Transport Conditions | Contact our team for specific details |
| Input Requirements | Genotyping and Methylation Profiling: The assay requires samples to be normalized to a concentration of 20-40ng/µL for whole blood DNA and 40-75 ng/µL for saliva DNA; in a volume of 30-50 µL. DNA should have an absorbance reading (260/280) of 1.7-2.0. Transcriptome Profiling:The assay requires samples to be normalized to a concentration of 50 ng/µL in a volume of 30 µL. |
| Workflow Time | 1-2 weeks (dependent on the project size) |
Find out more about our microarray services
Are you interested in learning more about the microarray services Sampled has to offer? Get in touch today to speak to an expert and receive a bespoke quote for your project!
