What is Sample Fractionation?
Fractionation involves the centrifugation of whole blood into separate fractions, usually to acquire serum or plasma.
For serum, a silicone gel within the centrifugation tube is typically used to keep the serum separated from the rest of the sample upon centrifugation. Unclotted blood separates into plasma, buffy coat (containing white blood cells), and red blood cells upon centrifugation without using a silicone gel.
Serum and plasma are both light-yellow in color but differ in their composition and suitability for downstream applications. Serum is the liquid that remains when clotted blood is centrifuged and plasma is the liquid that remains when unclotted blood is centrifuged as mentioned above.
Here at Sampled, we are fully equipped to rapidly fractionate a large quantity of whole blood samples ensuring high accuracy and fast turnaround times.
Why Use Sampled’s Fractionation Service?
Our team of technical experts at Sampled has vast experience in fractionating whole blood samples. Our high-end instrumentation means your samples will always be processed efficiently and accurately.
To ensure high quality we always isolate plasma within 48 hours of receiving it at our facility. Our process is fully automated which means we minimize handling and potential damage to your sample. Sampled’s robust storage and analysis facilities mean your sample can be processed immediately in-house without the need for further shipping.
Sampled can perform quality control on serum and plasma depending on our client’s requirements. We perform visual color grading of plasma, and analytical control using a Lunatic instrument from Unchained Labs which tests levels of bilirubin and hemoglobin, as well as the turbidity of the sample.
Fractionation allows for the isolation of blood plasma and serum which are central to many diagnostic and research applications.
- Disease identification
- Drug discovery
- Clinical trials
- Research studies
- Biomarker discovery
- Population health studies
- Disease modeling
- Development modeling
How Does Fractionation Work?
All samples we receive must arrive at room temperature. If they arrive frozen, subsequent thawing causes hemolysis which makes fractionation impossible. We also recommend sending us whole blood instead of plasma since frozen plasma is highly susceptible to degradation upon thawing. Upon receipt, the whole blood sample is maintained at room temperature and processed within 48 hours.
Whole blood fractionation is performed by an automated instrument operated by one of Sampled’s technical experts. Our Hamilton easyBlood STARlet™ liquid handling platform recognises the different layers of fractionated blood which enables it to safely aliquot the serum or plasma. Sampled’s technical expertise and high-end instrumentation means your samples are processed quickly and safely.
After fractionation, the sample can be sent immediately for analysis, storage, or shipping to the client or a third party chosen by the client. Fractionation of whole blood is an essential prerequisite for analyzing cell-free DNA.
What Are the Input Requirements for Fractionation?
The input requirement for fractionation is whole blood sent at room temperature.
Find out more about our fractionation services
Interested in expediting your whole blood fractionation? Get in touch with one of Sampled’s experts today to discover how we can meet your whole blood fractionation needs.