Sequencing
Sampled offers diverse sequencing services, including whole genome sequencing (WGS), whole exome sequencing (WES), targeted sequencing, Sanger sequencing, RNA sequencing, methylation sequencing, and advanced proteomics for comprehensive genomic and epigenetic analyses.
What Are Sampled’s Sequencing Services?
Sampled’s sequencing services are central to what we do as the world’s only Global Integrated Analytical Biorepository. Our sequencing services give our clients invaluable insights into their samples, expediting academic, industrial, and clinical research.
Whole-Genome Sequencing
Sampled offers comprehensive sequencing of your genome of interest, tailored to your needs. Our WGS services are customizable for various sample types and experimental requirements.
Sampled’s team has vast experience handling a variety of sample types, ensuring high-quality DNA extraction and QC for WGS. The next step is creating reliable, scalable libraries for analysis through fragmentation, end-repair, and covalent linking of genomic DNA (gDNA) to adapters. For long- or short-read sequencing, we use PacBio‘s Revio and Illumina‘s NovaSeq systems, known for their robustness and high throughput capacities. Data analysis includes further QC steps and can be performed in-house, or raw data can be sent to our clients for analysis.
Whole-Exome Sequencing
For a complete picture of the coding sequence of your genome of interest, Sampled offers bespoke WES services specific to your sample type and clinical/experimental needs.
Sampled excels in WES sample preparation, handling diverse sample types. QC checks ensure only high-quality DNA is processed. Library preparation includes genome fragmentation and targeted capture, emphasizing protein-coding regions for sequencing. We use state-of-the-art equipment, such as the NovaSeq 6000 system, for high-quality, high-throughput sequencing. Rigorous quality control analysis guarantees fit-for-analysis data, and clients can opt for customizable in-house bioinformatic analysis by our expert team. Sampled is ready to maximize the potential of your data, whether for rare variations or clinical sample analysis.
Targeted Sequencing
Our platform allows you to zoom in on specific genomic regions, providing detailed and accurate insights. Our expert team assists in experiment design, execution, and analysis.
Sampled processes diverse clinical and research samples, ensuring high-quality DNA extraction with stringent quality checks. Library preparation involves DNA fragmentation, end-repair, and DNA linkage to adapters for sequencing. Sequencing options for targeted, client-specified regions, include Illumina’s NovaSeq 6000, NovaSeq-X, and PacBio HiFi systems, chosen to suit project needs. The final step involves data analysis with QC checks. Data can be sent to clients or analyzed by Sampled’s in-house bioinformaticians, guaranteeing unbiased, high-quality analysis for optimized data utilization.
Sanger Sequencing
For precise detection of small DNA variants, we employ the tried and trusted Sanger sequencing method. Our platform combines high capacity with expert analysis, ensuring fast and accurate results.
After quality control checks, pre-extracted DNA is sent to the Sanger sequencing team, or DNA is extracted from raw samples. Following QC, DNA is normalized, and a PCR amplification is performed. The product undergoes another PCR reaction with fluorescently labeled modified DNA bases. Sequencing is done using Applied Biosystems machines. Analysis by two in-house experts ensures accurate results, reported through SampledSphere for easy client access and streamlined communication.
RNA Sequencing
With RNA sequencing, our clients get a detailed snapshot of gene activation in their sample. Sampled offers total RNA sequencing and full transcriptome profiling for streamlined research and diagnostics.
Sampled handles clinical and research samples with RNA as the starting material, either extracted in-house or provided by clients. Robust RNA QC is performed before further processing. Library preparation entails fragmenting RNA, followed by end repair, polyadenylation, reverse transcription, and barcoding with DNA adapters. Sequencing options are discussed with clients to align with the experiment’s specific goals, with partnered 10x Genomics and Illumina as potential platforms. Data analysis includes further QC and offers clients a range of expert analyses, ensuring data reliability and robust conclusions.
Methylation Sequencing
Analyze the methylation status of your genome of interest with our expert support, untangling the complex epigenetics of your sample.
Sampled’s methylation sequencing process begins with DNA extraction followed by stringent QC analysis. Library preparation involves genome fragmentation and adapter linkage. Our sequencing platform options include Illumina’s NovaSeq 6000 and PacBio’s 5-base HiFi platform for WGS and simultaneous methyl-cytosine detection. Robust QC ensures data quality, and clients can opt for their own in-house analysis or outsource it to Sampled’s expert bioinformaticians, who maintain the highest global standards.
Protein Biomarker Discovery
Discover and quantify novel protein biomarkers using our advanced proteomics service, featuring top-of-the-range workflows. Sampled ensures the best proteomics results for any sample type.
Sampled handles various samples for protein biomarker discovery, either provided by clients or isolated from complex samples by in-house experts. QC analysis ensures sample quality for further analysis. Olink’s Proximity Extension Assay employs three main steps: immune reaction, amplification, and next-generation sequencing to quantify protein levels. Data analysis is performed by expert bioinformaticians, with the option for clients to conduct their own analysis. All data is made available to clients in their preferred format on SampledSphere, our client-facing server.
Why Use Sampled’s Sequencing Services?
Expertise
At Sampled, our sequencing services are underpinned by a deep reservoir of expertise. Our team of seasoned experts is dedicated to providing comprehensive genetic, epigenetic, transcriptomic, and proteomic analysis, in addition to robust support for your research projects. We understand that the accuracy and precision of sequencing results are paramount, and our experienced professionals ensure that you receive the highest quality data, regardless of the sequencing service you choose.
Quality Control
Quality control is the cornerstone of our sequencing services. For all techniques, Sampled places a strong emphasis on quality control procedures to guarantee the reliability and accuracy of the data our clients receive.
Partnered with Global Leaders
Sampled is proud to collaborate with global leaders in the sequencing field, such as 10x Genomics, Illumina, PacBio, and Olink. These partnerships allow us to harness advanced sequencing technologies and innovations, including single-cell sequencing capabilities, which are invaluable for your research projects. With our partnerships, you can be confident that you are leveraging the very best in sequencing technologies to drive your discoveries.
Cost-Efficient
We understand the importance of cost-efficiency in research. Our targeted sequencing services, including WES, provide a cost-effective alternative to WGS that may be suitable for certain applications. By focusing on the specific genomic regions of interest, we can help you save on expenses if necessary while delivering high-quality and reliable data that meets your research needs.
Streamlined
At Sampled, we recognize the value of streamlined processes in sequencing. As the world’s only GIAB, our platforms are designed to streamline sample processing and analytical workflows, reducing turnaround times and maintaining sample integrity throughout the analysis pipeline. This efficiency ensures that you receive your results promptly, allowing you to move forward with your research without unnecessary delays.
Data Analysis Support
From the initial sequencing steps to the final data analysis, Sampled is committed to supporting your research journey. Our team of experienced bioinformaticians provides expert data analysis support, ensuring your results are robust, reliable, and meet the highest scientific standards. With our support, you can have confidence in the accuracy and significance of your research findings.
Sequencing Applications
Sampled’s robust sequencing platforms support a huge variety of applications from academic, industry, and clinical research programs. These include:
- Disease identification
- Diagnostics
- Drug discovery
- Pharmacogenomics
- Clinical trials
- Research studies
- Biomarker discovery
- Population health studies
- Novel genome assembly
- Agrigenomics
- Disease modeling
- Development modeling
- Drug testing
Sequencing Input Requirements
At Sampled, our experts are prepared to process a vast array of sample types from species including, but not limited to, human, mouse, and rat. This includes:
- Blood
- Plasma
- Serum
- Saliva
- Tissue biopsies
- Hair
- Nail
- Stool
- Urine
- Semen
- Cell line samples/pellets
- Protein samples
- Isolated nucleic acids (DNA/RNA)
- Buccal swabs
Find out more about our sequencing services
Interested in discovering more about your sample through an in-depth sequencing approach? Get in touch with one of Sampled’s experts today to discover how our vast sequencing platform can help you achieve your research goals.
