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iPSC Differentiation into Neuronal Cells
iPSCs can be used as powerful tool in regenerative medicine, drug discovery, therapeutic disease modelling, and to study development.
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How does the service work?
We are happy to announce that at Sampled, in addition to iPSC reprogramming and gene editing services, now offer iPSC-derived neuronal progenitor cell (NPC) differentiation services. NPCs are a progenitor population of cells that can differentiate into neuronal populations and can be used for studying physiology, disease phenotypes and drug testing.
Using a robust and reproducible method developed by Dr. Sally Temple at NeuroCell, iPSC-derived NPCs are generated in culture using guidance factors such as dual SMAD inhibition, to recapitulate the development of the forebrain and create NPCs that can be differentiated into forebrain neurons.
NPCs can be requested 3 ways
1
NPCs from a limited number of disease phenotypes are available off the shelf (Table 1)
2
iPSCs available from the NINDS Cell and Human Data Repository (NHCDR; found at stemcells. nindsgenetics.org) and the NeuraCell Core Facility (neuracell. org/cell-supply/stem-cells) can be used to generate NPCs.
3
Arrangements can be made for clients to provide iPSC of interest.
Tau Consortium iPSC lines
Table 1: Select Tau Consortium iPSC lines already validated for NPC production (see Karch et al, 2019)
| Cell Line | Mutation | Genotype |
|---|---|---|
| F11362.1 | R406W | WT/R406W |
| F11362.1d1C11 | R406W | WT/WT |
| F11362.1d1F10 | R406W | WT/R406W |
| F12436.3 | P301L/S | WT/WT (P301L family) |
| F12442.5 | PSEN1 | WT/WT |
| FTD-38-C1 | A152T | WT/A152T |
| FTD-T-C2 | A152T | WT/A152T |
| GIH-131-C1 | PSP | PSP |
| GIH-131-C2 | PSP | PSP |
| GIH-143-C3 | R406W | R406W homozygote |
| GIH-161-C1 | WT | WT/WT |
| GIH-161-C3 | WT | WT/WT |
| GIH-161-C4 | WT | WT/WT |
| GIH-6-C1d1A02 | V337M | WT/V337M |
| GIH-6-C1d1E11 | V337M | WT/WT |
| GIH-7-C2d2A01 | V337M | WT/V337M |
| GIH-7-C2d2B12 | V337M | WT/WT |
| GIH-7-C3 | V337M | WT/V337M |
| GIH-92-C1 | PSP | PSP |
| GIH-92-C2 | PSP | PSP |
| GIH-92-C3 | PSP | PSP |
| TAU-1225-C3 | A152T | WT/A152T |
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All NPCs can be shipped globally. If you would like to get in touch with us, please use the form below:
